Browsing by Author "Iweala, Emeka E.J."

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    Evaluation of Analgesic Property of Silver Nanoparticles Synthesized Using Mitracarpus scaber Aqueous Extract
    (Materials Science Forum (Volume 1148), 2025-05) Adesipe, Temitayo; Iweala, Emeka E.J.; Adebayo, Abiodun H.
    Pain is one of the main causes for which people seek medical attention. Strong, long-lasting, safe analgesics are therefore still needed in the medical industry to treat a wide range of painful disorders, despite recent advancements in pain treatment. The current work is intended to assess the analgesic properties of silver nanoparticles (AgNPs) synthesized using Mitracarpus scaber aqueous extract. Aqueous extraction of Mitracarpus scaber whole plant was done and employed to make and cap AgNPs using 0.1M AgNO3 solution. The produced AgNPs were examined using a variety of methods, such as Fourier Transform Infrared Spectroscopy (FTIR), UV VIS, and Transmission Electron Microscopy (TEM). Acetic acid writhing and hot plate tests were then used to investigate the analgesic activity of various doses of the biosynthesized AgNPs. AgNPs production was detected by UV-vis spectroscopy at λ max = 425 nm. The biosynthesized AgNPs had a size range of 4-5 nm, were crystalline and spherical in shape, according to TEM investigations. FTIR analysis on the other hand revealed the involvement of phenyl, hydroxyl, carbonyl, and aliphatic carbon-hydrogen bonds in the AgNPs' production. The biogenic silver nanoparticles showed the highest reduction of writhes and pain at the doses of 100 and 50 mg/kg b.w. respectively, which were significantly higher than the analgesic activity elicited by Diclofenac (50 mg/kg b.w.) for both assays. The present work therefore concludes that biogenic silver
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    Lentinus squarrosulus: Nutritional composition, phytochemistry, healthpromoting activities and toxicity profile
    (Food and Humanity Volume 2, 2024-05) Ugbogu, Eziuche A.; Dike, Emmanuel D.; Okoro, Benedict C.; Adurosakin, Oluwapelumi E.; Ibe, Chibuike; Uche, Miracle Ebubechi; Nosiri, Chidi I.; Agim, Cosmas; Cosmas, Ezeibe C.; Rahman, Md. Atiar; Iweala, Emeka E.J.
    Lentinus squarrosulus (Mont) is a nutritious and therapeutic edible mushroom with a tough fruiting body. This study aims to provide comprehensive and up-to-date literature on the nutritional composition, biologically active compounds, and health-promoting effects of L. squarrosulus. Databases such as ScienceDirect, Wiley, Springer, and PubMed were used for the material search, and only papers published in English were considered. Biologically active compounds contained in L. squarrosulus are 1- tetradecene, fumaric acid, 9-eicosene, phytol, octahydropyrrolo[1,2-a]pyrazine, 3- trifluoroacetoxypentadecane F, 2(5 H)-furanone, methyl 2-oxo-1-pyrrolidine acetate, ergosterol, 9-octadecenoic acid (Z)-, methyl ester, hexadecanoic acid, methyl ester, pentadecanoic acid-methyl ester, albuterol, oleic acid, gallic acid, protocatechuic acid, gentisic acid, vanillic acid, caffeic acid, p-hydroxybenzoic acid, p-coumaric acid, cinnamic acid, catechin, tannic acid, quercetin, isoquercetin, kaempferol, and eriodictyol. Biochemical studies showed that L. squarrosulus possesses antimicrobial, antiulcer, anticancer, antioxidant, gastroprotective, and antidiabetic activities. This study has shown that L. squarrosulus has a therapeutic effect against various of diseases and can be used as a raw material for the development of therapeutic products. However, clinical studies are needed to prove the efficacy of L. squarrosulus for therapeutic purposes.
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    Tumor-normal sequencing reveals novel TP53 germline and clinically actionable somatic mutations in Nigerian breast cancer patients
    (Cancer Genetics Volumes 300–301, 2026-01) Onyia, Abimbola F.; Lawal, AbdulRazzaq; Ogo, Chidiebere; Nkom, Ebenezer S.; Lasebikan, Nwamaka N.; Ayegbusi, Olaitan T.; De Campos, Opeyemi C.; Rotimi, Oluwakemi A; Oyelade, Jelili O.; Aliyu, Usman M.; Iweala, Emeka E.J.; Rotimi, Solomon O
    Purpose Disparities in the care and management of breast cancer (BC) contribute to poor outcomes and limited access to precision oncology in Nigerian patients. Existing studies on Nigerian patients have largely been conducted abroad, restricting their direct application to local healthcare. This study addresses this gap through a locally led investigation of germline and somatic mutations using tumor-normal paired sequencing. Methods Forty-two female BC patients were recruited from teaching hospitals between January and April 2024. DNA was extracted from blood and matched fresh-frozen tumor tissue. Targeted sequencing of 50 cancer-related genes was performed with the Illumina AmpliSeq Cancer Hotspot Panel and MiSeq platform. Germline and somatic variants were identified through matched normal filtering, with oncogenic significance assessed using the ESCAT/ESMO Tier classification. Visualization was performed in R (v4.4.2) using the maftools package. Results A germline TP53 pathogenic variant, TP53 c.694dupA (p.Ile232Asnfs) was identified in a 35-year-old triple-negative BC patient with recurrent metastatic disease, representing its first report as a germline alteration. Additionally, eighteen oncogenic/likely oncogenic somatic variants were detected, nine of which were actionable (Tier IIII). EGFR amplification was found in 7 % of patients, alongside copy number losses in genes including CDKN2A and KIT. Conclusion This study demonstrates the feasibility of localized tumor-normal sequencing in Nigerian BC patients, revealing actionable variants with clinical relevance. These findings highlight the need to integrate genomic profiling into routine cancer care and establish molecular tumor boards to advance precision oncology in Nigeria.