EFFECTS OF JOBELYN® AND RESVERATROL ON BISPHENOL-A-INDUCED MALE REPRODUCTIVE DYSFUNCTIONS IN WISTAR RATS

Abstract

Recent years have shown a significant deterioration in sperm quality among men, largely attributed to prolonged exposure to environmental endocrine disruptors. Bisphenol-A (BPA), a prominent disruptor, contributes to this issue by inducing oxidative stress and disrupting the hypothalamus-pituitary-gonadal axis. The study aims to examine the effects of Resveratrol and Jobelyn on reproductive dysfunction in BPA-induced male rats. BPA was exposed to forty-two male rats, grouped into six. Every group—aside from the control, were exposed to 200 mg/kg of BPA. The treatment groups included: Group 3, which received 20 mg/kg of Resveratrol; Group 4, treated with 5 mg/kg of clomiphene citrate; Groups 5 and 6, administered 100 mg/kg and 200 mg/kg of Jobelyn, respectively; and Group 7 received a combined dose of 200 mg/kg of Jobelyn and 20 mg/kg of Resveratrol. This experiment was conducted over a duration of 28 days. The testicular, and epididymal antioxidant enzymes (glutathione-S-transferase and superoxide dismutase) and the malondialdehyde (MDA) level were evaluated. The sperm quality and serum hormonal (luteinizing hormone, testosterone, and follicle stimulating hormone) concentration were also evaluated. The results revealed that exposure to BPA significantly (p<0.05) reduced the sperm quality, activity of antioxidant enzymes, and hormonal levels compared to the control group, while it caused an increase in malondialdehyde (MDA) levels. Treatment with Jobelyn and Resveratrol significantly (p<0.05) improved sperm quality and hormonal levels, enhanced antioxidant enzyme activity, and reduced malondialdehyde (MDA) levels in comparison to BPA-induced rats. The histopathology analysis showed no structural or cellular alteration to the testes and epididymis. This study suggests that Jobelyn and Resveratrol may be beneficial in managing hormonal imbalances caused by oxidative stress in men.