An optimized colony forming assay for low-dose-radiation cell survival measurement
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The aim of this study is to develop a simple and reliable method to quantify the cell survival of low-dose
irradiations. Two crucial factors were considered, the same number of cells plated in each flask and an
appropriate interval between cell plating and irradiation. For the former, we optimized cell harvest with
trypsin, diluted cells in one container, and directly seeded cells on the bottom of flasks in a low density
before irradiation. Reproducible plating efficiency was obtained. For the latter, we plated cells on the
bottom of flasks and then monitored the processing of attachment, cell cycle variations, and the plating
efficiency after exposure to 20 cGy of X-rays. The results showed that a period of 4.5 h to 7.5 h after
plating was suitable for further treatment. In order to confirm the reliability and feasibility of our
method, we also measured the survival curves of these M059K and M059J glioma cell lines by following
the optimized protocol and obtained consistent results reported by others with cell sorting system. In
conclusion, we successfully developed a reliable and simple way to measure the survival fractions of
human cells exposed to low dose irradiation, which might be helpful for the studies on low-dose
radiation biology.
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QC Physics