Coexistence of Aflatoxicosis with Protein Malnutrition Worsens Hepatic Oxidative Damage in Rats
| dc.creator | Rotimi, Oluwakemi A, Rotimi, Solomon O, Oluwafemi, Flora, Ademuyiwa, Oladipo, Balogun, Elizabeth A | |
| dc.date | 2016 | |
| dc.date.accessioned | 2025-03-28T17:15:54Z | |
| dc.description | To investigate the effects of the coexistence of aflatoxin B1 (AFB1) and protein malnutrition in rat liver, weanling rats were fed either normal protein diet (20% protein), low-protein (PEM) diet (5%), normal protein diet + 40 ppb AFB1, or low-protein diet + 40 ppb AFB1. After 8 weeks, biomarkers of hepatic functions and oxidative stress, caspase-3 activity, and tumor suppressor protein 53 (p53) were determined spectrophotometrically. Randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was employed to determine genomic alterations among the groups. Coexistence of aflatoxicosis and PEM significantly decreased glutathione, glutathione-S-transferase, glutathione peroxidase, and superoxide dismutase, while it increased peroxidase and catalase. RAPD-PCR showed genomic alterations that were associated with significant increases in p53 level and caspase-3 activity in rats fed PEM diet + AFB1. In conclusion, the coexistence of aflatoxicosis and protein malnutrition induced oxidative stress with concomitant genomic alterations in the liver of weanling rats. | |
| dc.format | text/html | |
| dc.identifier | http://eprints.covenantuniversity.edu.ng/5903/ | |
| dc.identifier.uri | https://repository.covenantuniversity.edu.ng/handle/123456789/35266 | |
| dc.language | en | |
| dc.publisher | Wiley Online Library | |
| dc.subject | Q Science (General) | |
| dc.title | Coexistence of Aflatoxicosis with Protein Malnutrition Worsens Hepatic Oxidative Damage in Rats | |
| dc.type | Article |