Expression of the C-terminal family 22 carbohydratebinding
module of xylanase 10B of Clostridium
themocellum in tobacco plant

dc.creator	Obembe, Olawole O.
dc.date	2009-11
dc.date.accessioned	2025-03-06T10:20:00Z
dc.description	Carbohydrate-binding modules have been shown to alter plant cell wall structural architecture. Hence, they have the potential application of being used to engineer the plant to produce tailor-made natural fibers in the cell wall. The Clostridium thermocellum xylanase, Xyn10B, contains two CBMs that belong to family 22 (CBM22). The C-terminal CBM22-2 of the glycoside hydrolase (GH) 10 had been characterized to interact with xylan, a major hemicellulosic component in the secondary cell wall of plants. In this work, the expression of the CBM22-2 in transgenic tobacco plants was evaluated. Histological examinations of the transgenic stems did not reveal marked cell wall phenotype. In addition, there were no observable changes in the height or the appearance of the transgenic plants expressing the CBM22-2 module. The results indicate that the family 22 carbohydrate binding module is not a potential candidate for use in in planta modification of the cell wall.
dc.format	application/pdf
dc.identifier	http://eprints.covenantuniversity.edu.ng/363/
dc.identifier.uri	http://itsupport.cu.edu.ng:4000/handle/123456789/28989
dc.language	en
dc.publisher	Academic Journals
dc.subject	QK Botany
dc.title	Expression of the C-terminal family 22 carbohydratebinding module of xylanase 10B of Clostridium themocellum in tobacco plant
dc.type	Article

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