EFFECTS OF INTERLEUKIN- 6 MEDIATED INFLAMMATION ON TELOMERASE EXPRESSION IN MALARIA PATIENTS

Abstract

Malaria remains a major global health burden that mostly affects young children in the African region, which has been associated with cellular aging and immune system exhaustion that is potentially mediated through telomere shortening and altered telomerase activity. The influence of malaria on the catalytic subunit hTERT, and how it modulates telomerase expression, in relation to the proinflammatory cytokines such as interleukin-6 (IL-6) and interferon-gamma (IFN-γ) is yet to be established. This study, therefore, aimed to explore the relationship between IL-6, IFN-γ levels, and hTERT gene expression in individuals with malaria infection. Ethical approval was obtained from the Covenant Health Research Ethics Committee (CHREC) before commencement of the study. A total of 50 malaria-infected samples were collected from ACE-Medicare and Covenant University Medical Center. Plasma generated from venous blood samples (5 ml) was separated by centrifugation, collected, and stored at –80 °C for subsequent cytokine analysis. Total RNA was extracted for cDNA synthesis and RT-qPCR-based quantification of hTERT expression. RNA concentration, integrity, and purity were analyzed using a Nanodrop spectrophotometer. A portion of the plasma (100 μl) was used for cytokine analysis using human IL-6 and IFN-γ using ELISA technique. Interleukin-6 levels (17.47 ± 25.11 pg/ml) were significantly higher (p<0.05) in the case compared to the control group (0.54 ± 0.46 pg/ml). The interferon gamma levels (117.74 ± 51.62 pg/ml) in the case group showed no significant difference (p>0.05) compared to the control group (104.50 ± 55.23 pg/ml). The Ct values of the hTERT gene expression were 33.38±4.48 in malaria patients in Nigeria, which is a possible standard range. For the first time, this study reports hTERT gene expression levels in Nigerian malaria patients and IL-6 as potential biomarkers for monitoring malaria progression, thereby providing a valuable tool for precision malaria treatment in Nigeria