2025-03-27https://repository.covenantuniversity.edu.ng/handle/123456789/34383An investigation was made on the improved production and r~covery of the new and potent immunos..:opressiv~ i)eptide, cyclosporine (cyclo~porin A, Cy A) from a glucose-adapted isolate_ of the fungus i. ~ J/C,...ladiurn inflaturn. Among the amino acid members in the Cy A molecule, L-leucine and especially L-valin~:t had a strong positive effect on production of Cyclosporine. ·When other conStituent amino acids were added with L-valine, negative effe.cts were obseiVed. Optimal time of L-valine was 48 . ' . tus. In the case of semisynthetic medium and its optimal amount-was 4·gramslliter (g/1). Considerable improvements in the recovery of this drug were, ~~ieved by modifying ul~ available extraction pro~edure with the addition of surfactant, or With alkali apqilion followed by moqerate heat treatmEmtapplication/pdfQ Science (General), QH301 BiologyArticle