Browsing by Author "Tebamifor, Mercy Eyitomi"

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    Surveillance of Wolbachia infection in mosquito species in Ota, Ogun State, Nigeria
    (Discover Applied Sciences, 2025-03-27) Tebamifor, Mercy Eyitomi; Cleanclay, Wisdom D.; Mamudu, Collins Ojonugwa; Ogunlana, Olubanke Olujoke
    Introduction In light of climate change, proliferation of mosquito-borne diseases like dengue and malaria is a mounting concern, driven by expanding mosquito populations as a result of favorable environment for their survival. Addressing public health challenges caused by mosquitoes demands constant innovation and sustainable solutions. Objective This study responds to recent reports of Wolbachia infections in West African mosquito species, suggesting their potential as biocontrol agents for disease vectors. We seek to detect the presence of Wolbachia pipientis in different mosquito species in Ota and identify mosquito species present in the area. Method We conducted a comprehensive mosquito larval surveillance in Ota, Ogun State, Nigeria using a systematic stratified random sampling method from November 2022 to March 2023 to assess mosquito species distribution and Wolbachia infection. During this period, we surveyed mosquito larvae in various sites, rearing them to adulthood. We meticulously identified species, sex, and collection locations then, stored specimens at − 20 °C. Sodium chloride precipitation protocol was employed to extract DNA from the mosquitoes individually. Polymerase chain reaction (PCR) analysis was carried out using one to one point five microliter of DNA, with distilled water as negative control. Results Out of 1265 emerging young adult mosquitoes, 62.1% were females, while 37.1% were males. Aedes species constituted 22.2%, Anopheles 37.2%, and Culex 40.6% of the population. DNA analysis identified Wolbachia infection in Ae. albopictus and Ae. aegypti, with wsp gene sizes ranging from 590 to 632 bp, confirming Wolbachia presence by sequencing. Conclusion Our study is the first report on Wolbachia presence in Aedes sp within this region, which suggests that this mosquito species is a less likely vector for dengue virus and other related infectious agents. The study highlights the importance of continuous mosquito population and breeding site monitoring for potential biocontrol interventions against disease vectors.