Programme: Applied Biology

Permanent URI for this collectionhttp://itsupport.cu.edu.ng:4000/handle/123456789/28776

Here you will find works strictly related to Applied Biology

Browse

Filter results by typing the first few letters
Now showing 1 - 1 of 1
  • No Thumbnail Available
    Item
    EXPRESSION PROFILES OF CYTOCHROME P450 GENES ASSOCIATED WITH PERMETHRIN RESISTANCE IN Anopheles gambiae s.l. IN ADO-ODO OTA, OGUN STATE
    (Covenant University Ota, 2025-10) AINA, Motunrayo Oluwabunmi; Covenant University Dissertation
    Malaria remains a significant tropical public health threat, where resistance to insecticides constitutes a severe hindrance to the efficacy of its primary vector control methods. Routinely applied pyrethroid insecticides are increasingly facing resistance associated with the overexpression of cytochrome P450 genes in the Anopheles gambiae sensu lato, underscoring the urgent search into these associated genes. This study assessed the expression profile of cytochrome P450 genes associated with permethrin resistance in Anopheles gambiae sensu lato collected from three localities in Ado-Odo, Ota. Ethical approval was obtained from the Covenant Health Research Ethics Committee (CHREC). Based on the WHO standard, female adult An. gambiae larvae (n=300) were collected using the dipping method and reared into adults in the Insectary Laboratory. These laboratory-reared mosquitoes were phenotypically identified using microscopy and genotypically characterised using polymerase chain reaction (PCR) based on species-specific primers. Thereafter, a WHO susceptibility bioassay was conducted in vivo for mosquitocidal activity against these adult mosquitoes in four replicates at a 0.75% permethrin concentration each on day 3 post-adult emergence. The relative expression of the cytochrome P450 genes (CYP6M2 and CYP6P3) was carried out using the quantitative real-time PCR (qRT-PCR). Higher occurrence rate of An. gambiae sensu lato. was recorded in Nestle (80%)), Chelsea (78%)) compared Gasline (30%) localities of Ado-Odo. In vivo insecticide susceptibility testing revealed consistently low mortality rates across all the replicates, ranging from 20% to 32% indicating increased resistance to permethrin. Results of relative expression of cytochrome P450 genes showed higher fold changes in CYP6M2 ranging from 0.63 to 122.4 than in CYP6P3 0.63 to 34.39 across the tested mosquito replicates. Thus, this study has further emphasized the prevalence of An. gambiae sensu lato members in Ado-Odo, Ota. Additionally, the results of higher permethrin resistant and upregulation of CYP6M2 and CYP6P3 genes inform the imminent need for integrated resistance surveillance with newer vector management for improved malaria control.