Antibacterial Activity of Cell-Free Supernatants of Probiotic Lactobacillus against Bacterial Pathogens Associated with Vaginal Infections
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EBSCOhost'
Abstract
Description
Vaginal infections are common female disease conditions that account for the
prevalence of gynecological disorders which facilitate the increasing antimicrobial
resistance and failure of prevalent treatment choices. In this study, the
antibacterial activity of cell free supernatants (CFS) of probiotic Lactobacillus
obtained from ogi (fermented maize) was evaluated against bacterial pathogens
associated with vaginal infections. Bacterial pathogens isolated from high vaginal
(n=22) and endocervical swabs (n=18) were bio-typed and assayed for hemolytic
activity, biofilm production, antibacterial susceptibility pattern, and the CFS
antagonistic activity. The occurrence of the vaginal bacterial pathogens was
33.0% for Streptococcus spp. and 31.0% for Staphylococcus aureus, with more
than 70% resistance rates to amoxicillin, cefotaxime, imipenem/cilastatin,
nalidixic acid, nitrofurantoin, cefuroxime, ceftriaxone sulbactam, ampiclox,
cefixime and levofloxacin. More than 30% of the isolates produced biofilms. Of
the four identified probiotic strains, only CFS from L. plantarum and L.
acidophilus exhibited observable antagonistic reaction, with L. plantarum
showing higher antibacterial activity against Staphylococcus condimenti, and L.
acidophilus against Klebsiella pneumoniae. With the results of this study
revealing the antibacterial activity of probiotic Lactobacillus CFS against vaginal
bacterial pathogens, probiotic Lactobacillus can be suggested for use as
prophylactic and bioprotective agents in the therapeutic management of vaginal
bacterial infections and preservation of the vaginal microbiota
Keywords
QH Natural history, QH301 Biology, QR Microbiology