Influence of synthetic carbon grade on the metabolic flux of polyhydroxyalkanoate monomeric constitution synthesized by Bacillus cereus AAR-1
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Description
Carbon substrate is a pivotal factor influencing polyhydroxyalkanoate (PHA) properties
of varied industrial importance. Three synthetic sucrose samples with varying
manufacturing purity levels were selected as carbon substrates to synthesize diverse
PHAs using a wild-type Bacillus cereus AAR-1. Comparative monomeric analyses of the
extracted biopolymers revealed Poly (3-hydroxytetradecanoate) (P3HTD), Poly(3-
hydroxybutyrate-co-2-hydroxytetradecanoate) [P(3HB-co-2HTD)], and Poly(3-
hydroxybutyrate) (P3HB) with carbon elemental contents that ranged from 39 to 53 %
and no nitrogen detected. The decomposition temperature of [P(3HB-co-2HTD)] was
279 °C, indicating higher thermal stability than the individual monomeric units. Notably,
the homopolymer P3HTD exhibited an increased melting temperature of 172.4 °C and a
reduced crystallinity percentage (Xc % = 20.7 %), crucial properties for bioplastics and
medical sector applications. All the biopolymers displayed a low specific heat capacity
ranging between 0.03 and 0.05 J/g°C, suitable for applications such as thermal storage
materials and temperature-regulating textiles. The results suggest that different carbon
purity grades influenced homopolymer accumulated in Bacillus cereus AAR-1.
Keywords
RA Public aspects of medicine, TP Chemical technology