Reactive Sulphydryl Groups in Horse Carbonmonoxyhaemoglobin
No Thumbnail Available
Date
Journal Title
Journal ISSN
Volume Title
Publisher
Academic Journals
Abstract
Description
Background and Objective: Reactive sulphydryl groups in haemoglobin has been related with oxygen binding. In this study,
the researchers analyzed the reactive sulphydryl groups in horse (Equus ferus caballus) carbonmonoxyhaemoglobin.
Materials and Methods: Hemolysate gotten from fresh horse blood was converted to yield the carbonmonoxy derivative. It was then
separated via carboxymethylcellulose into major and minor fractions of haemoglobin. These fractions were titrated with Ellmanʼs reagent
(DTNB) and p-hydroxymercuri(II)benzoate (p-MB) stock solutions in increasing volumes. Results: Results showed that two sulphydryl
groups reacted with DTNB and p-MB in both major and minor haemoglobin fractions. p-MB is known to be more reactive with thiols than
DTNB, on the other hand the reactivity is the same with horse carbonmonoxyhaemoglobin. Conclusion: This study will enable a better
understanding as regards the kinetics and equilibrium behind this reaction.
Keywords
QR Microbiology