Reactivities of the sulphydryl groups of horse (Equus caballus) haemoglobin (1013.11)
| dc.creator | Omotosho, O. E., Chinedu, S. N., Omotosho, T. V., Okonjo, K. O. | |
| dc.date | 2014 | |
| dc.date.accessioned | 2025-03-28T17:03:51Z | |
| dc.description | The kinetics of the reaction of Ellman’s reagent (DTNB) with CysF9[93]5 sulphydryl group of the horse haemoglobins were studied at neutral and physiological pH (6.8 < pH > 7.6) ranges under pseudo first order conditions. The reaction is of first order with respect to the DTNB concentration. The reactions are pH dependent of the observed rate constant gave a complex trend. The observed rate shows that at neutral pH, the presence of inositol hexakisphosphate (inositol-P 6 ) increases the pseudo first order rate constant. For the first time, inositol-P 6 increases rate of forward reaction, kF at neutral pH values by increasing the mean value of the transition constant, K rt3 . The K rt3 for the haemoglobin without inositol-P 6 gave the value of 0.138 ± 0.1 while the haemoglobin in the presence of inositol-P 6 gave the K rt3 value of 0.325 ± 0.2. The results show that inositol-P 6 increases the relative population of the t tertiary conformation. So, it increases the reactivity of CysF9[93]5 by changing the relative distribution of two protein conformations | |
| dc.format | application/pdf | |
| dc.identifier | http://eprints.covenantuniversity.edu.ng/5684/ | |
| dc.identifier.uri | https://repository.covenantuniversity.edu.ng/handle/123456789/35060 | |
| dc.language | en | |
| dc.subject | QH301 Biology | |
| dc.title | Reactivities of the sulphydryl groups of horse (Equus caballus) haemoglobin (1013.11) | |
| dc.type | Article |
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