Assessment of Genetic Diversity among Selected Tomato (Solanum lycopersicum L.) Lines Based on SSR Markers
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Description
Genetic diversity in eight Nigeria, two Benin Republic and five United Kingdom cultivated tomato lines was
assessed using simple sequence repeat (SSR). Genotyping was carried out with 15 SSRs. Among the markers
used, 11 SSR markers were polymorphic. For the SSR analysis, the total number of polymorphic alleles was 33 ,
with a mean of 3.00 and the average polymorphic information content (PIC) was 0.36. The genetic diversity
within the lines was considerably moderate (0.31). The Estimates of Rogers ' distance varied from 0.03 to 0.66.
The 15 lines were clustered into two major groups and a singleton based on unweighted pair-group method with
arithmetic mean (UPGMA) cluster analysis of the SSR-based genetic distance (GD) estimates. Group I included
10 lines from all the geographical regions evaluated, and the group II consists of 4 lines which are essentially
from Nigeria. TM09 (UK line) grouped separately forming a singleton. Understanding the genetic diversity
among the tomato lines is a good starting point for establishing and maintaining collections, gem1plasm ba11ks
and proper characterisation of Nigerian tomatoes in order to preserve the genetic variability
Keywords
QH Natural history, QH301 Biology