VIRULENCE FACTORS AND ANTIMICROBIAL RESISTANCE PATTERN OF BACTERIAL ISOLATES FROM READY-TO-EAT WATERMELONS AND PINEAPPLES IN OTA
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Ready-to-eat (RTE) fruits are increasingly found in the markets as consumers demand fresh
fruits on-the-go to meet up with a fast-paced lifestyle. Despite their advantages, the fruits are
exposed to contamination due to the many processes they go through before they reach the
consumer. This creates conditions for pathogens to establish themselves on the surface and
within the pulp of fruits which may lead to illnesses on consumption. The aim of this study was
to examine retailed RTE fruits in Ota for bacterial contaminants, highlight their antibiotic
susceptibility patterns and detect selected virulence factors. RTE watermelons and pineapples
were sampled from vendors along strategic locations in Ota, Ogun State. The fruits were
analyzed using standard microbiological methods. Presumptive identification was by
biochemical tests, Kirby-Bauer disc diffusion method was used to determine their resistance
patterns against eight antibiotics. The isolates were screened for haemolytic activity, protease
activity, and biofilm formation. Molecular characterization was carried out using polymerase
chain reaction. The 16SrRNA gene of the isolates was amplified using the primer pair 27F-
5′- AGAGTTTGATCCTGGCT CAG -3’, and 1492R 5’- GGTTACCTTGTTACGACTT -3’.
to confirm isolates. The isolates were tested against eight antibiotics for their susceptibility and
screened for haemolytic activity, protease activity, and biofilm formation. The isolates
identified via Gram-staining and biochemical tests were Bacillus, Listeria, Sarcina,
Pseudomonas, Lactobacillus, Micrococcus, Staphylococcus, and Streptococcus species.
Bacillus sp. was detected in both pineapples and watermelons. The most active antibiotics with
their susceptibility rates were Ofloxacin (88%), Gentamicin (77%), and Erythromycin (33%).
A 100% resistance to Ceftazidime, Cefuroxime, Cloxacillin, Nitrofurantoin, and Cefixime was
observed. Staphylococcus was resistant to all the antibiotics. The isolates possessed at least one
virulence factor. Of the isolates, 38.9% were negative for biofilm formation, 50% were
proteolytic, and 61.1% showed either beta or alpha haemolysis. Bacteria with diverse
pathogenic factors are present in RTE fruits. It is recommended that actions which will reduce
the bacterial load on RTE fruits are enforced.
Keywords
QR Microbiology