Production of Polyclonal Antibody Against an Isolate of Yam-infecting Badnavirus from Nigeria

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dc.creatorEni, A. O., Hughes, J. d' A., Rey, M. E. C.
dc.date2010
dc.date.accessioned2025-03-26T12:36:19Z
dc.descriptionIntegrated viral sequences and high sequence variability among badnaviruses complicates the development of specific reliable molecular detection tests for yam-infecting badnaviruses. Thus Serological techniques are of notable importance for routine testing and monitoring of these viruses. The major limiting factor to the use of serological techniques is the limited availability of antibodies. Rabbit polyclonal antibody was produced against a purified preparation of a yam-infecting badnavirus from Nigeria. Antibody titre was determined by Protein-A sandwich (PAS) enzyme-linked immunosorbent assay (ELISA). The antibody produced had a titre of 1:1280 in PAS-ELISA and detected yam-infecting badnaviruses in infected yam leaves from Nigeria, Ghana, Benin and Togo. The suitability of the antibody for use in immunocapture polymerase chain reaction (IC-PCR) was evaluated. The antibody successfully trapped both Dioscorea alata bacilliform virus (DaBV) and Dioscorea sansibarensis bacilliform virus (DsBV) for IC-PCR detection. The antibody produced in this study will enhance certification of yam planting materials across West Africa and also facilitate the safe international movement of yam germplasm.
dc.formatapplication/pdf
dc.identifierhttp://eprints.covenantuniversity.edu.ng/2114/
dc.identifier.urihttps://repository.covenantuniversity.edu.ng/handle/123456789/31447
dc.languageen
dc.subjectQR Microbiology
dc.titleProduction of Polyclonal Antibody Against an Isolate of Yam-infecting Badnavirus from Nigeria
dc.typeArticle

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