Methods of Extraction and Antimicrobial Susceptibility Testing of Plant Extracts
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Plant extracts are widely used in pharmaceutical, food and cosmetic industry; however the effect of
conventional methods of extraction described in this paper as cold and soxhlet method has not been
investigated. Antimicrobial activity of Anchomanes difformis (Blume) Engl. leaf extracts have been described
comparing cold and soxhlet method of extraction. The ammmt of extractable plant extract/phytochemicals,
the solubility of the extracts and the susceptibility of the test organism to the extract had a correlation with the
extraction methods. Cold extract had wider zones of inhibition and activity than soxhlet extract. Though there
was no significant difference (P>0.05) between cold and soxhlet extracts on the entire test organisms,
differences exist when compared individually. This paper revealed cold method of extraction as a better
alternative to soxhlet method in antimicrobial susceptibility assay. However, the decision on the methods of
extraction to be used could also be dependent on the ammmt of certain phytochemicals required and the
solubility of the extracts in solvents after extraction.
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QH301 Biology