Strategies For Enhanced Production And Recovery Of Cyclosporine
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An investigation was made on the improved production and r~covery of the new and potent
immunos..:opressiv~ i)eptide, cyclosporine (cyclo~porin A, Cy A) from a glucose-adapted isolate_ of
the fungus i. ~ J/C,...ladiurn inflaturn. Among the amino acid members in the Cy A molecule, L-leucine
and especially L-valin~:t had a strong positive effect on production of Cyclosporine. ·When other conStituent
amino acids were added with L-valine, negative effe.cts were obseiVed. Optimal time of L-valine was 48
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tus. In the case of semisynthetic medium and its optimal amount-was 4·gramslliter (g/1). Considerable
improvements in the recovery of this drug were, ~~ieved by modifying ul~ available extraction pro~edure
with the addition of surfactant, or With alkali apqilion followed by moqerate heat treatmEmt
Keywords
Q Science (General), QH301 Biology